A new methodology has been proposed to measure optical properties of homogeneous tissue where a laser beam is used to induce heat to a tissue. The induced heat increased the temperature inside the tissue, which is detected by a thermocouple. These readings are compared with that obtained from the solution of the finite element solution that used iterative values of optical properties in determining temperature distribution. The two temperature distributions are used to determine tissue optical properties using the Levenberg-Marquardt iteration. An accurate result is obtained in determining absorption coefficient and reduced scattering coefficient. The work is extended to obtain three parameters (i.e., absorption coefficient, scattering coefficient, and anisotropy). The only limitation is that the temperature readings have to be measured with a high-accuracy thermocouple (i.e., less than 0.4% of maximum-recorded temperature).
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