Rapidly monitoring the quality of flavoring essence based on microwave-induced plasma ionization mass spectrometry and multivariate statistical analysis.

Microwave-induced plasma ionization mass spectrometry (MIPI-MS) combined with multivariate statistical analysis was first applied to rapidly monitor the quality of tobacco flavoring essence. A small isolation and reaction chamber was set up between the ion source and the injection port of mass spectrometer to effectively eliminate the interference of external environment in the process of analyzing samples. The improved experimental apparatus (MIPI-MS) could achieve online and high-throughput analysis, with minimal sample preparation steps. Further, two types of tobacco flavoring essences with the similar appearance and physicochemical parameters were employed to verify the usability of the promising method in the field of quality monitoring. Firstly, the mass spectral fingerprint of each essence was established by the improved MIPI-MS method within 2 min. Then, two multivariate statistical processes were carried out to analyze mass spectral data. The similarity results indicated that the thresholds of tobacco flavoring essences from different batches were 1.512 and 2.638, respectively. The first three principal components of the established PLS-DA described 93.6% of the total variability, and provided a visualized comparison for the two types of flavoring materials. Finally, the adulterated samples were successfully distinguished by employing the two multivariate statistical processes.