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The Inescapable Effects of Ribosomes on In-Cell NMR Spectroscopy and the Implications for Regulation of Biological Activity. | LitMetric

The Inescapable Effects of Ribosomes on In-Cell NMR Spectroscopy and the Implications for Regulation of Biological Activity.

Int J Mol Sci

Department of Chemistry, University at Albany, State University of New York, 1400 Washington Ave., Albany, NY 12222, USA.

Published: March 2019

AI Article Synopsis

  • * It highlights that quinary interactions between labeled proteins and RNA can lead to unexpected large molecular weights in NMR spectra, which can be analyzed through specific techniques like CRINEPT, HMQC, and TROSY.
  • * The study shows that ribosomal binding antibiotics can change the structure and activity of proteins by altering their interactions with ribosomes and mRNA, suggesting that ribosomes play a key role in regulating biological functions.

Article Abstract

The effects of RNA on in-cell NMR spectroscopy and ribosomes on the kinetic activity of several metabolic enzymes are reviewed. Quinary interactions between labelled target proteins and RNA broaden in-cell NMR spectra yielding apparent megadalton molecular weights in-cell. The in-cell spectra can be resolved by using cross relaxation-induced polarization transfer (CRINEPT), heteronuclear multiple quantum coherence (HMQC), transverse relaxation-optimized, NMR spectroscopy (TROSY). The effect is reproduced in vitro by using reconstituted total cellular RNA and purified ribosome preparations. Furthermore, ribosomal binding antibiotics alter protein quinary structure through protein-ribosome and protein-mRNA-ribosome interactions. The quinary interactions of Adenylate kinase, Thymidylate synthase and Dihydrofolate reductase alter kinetic properties of the enzymes. The results demonstrate that ribosomes may specifically contribute to the regulation of biological activity.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471074PMC
http://dx.doi.org/10.3390/ijms20061297DOI Listing

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