Background And Objectives: One of the most important antibiotic-resistant bacteria is methicillin-resistant (MRSA) biofilm that has caused significant problems in treating the patients. Therefore, the aim of this study was to evaluate the levels of expression of genes involved in biofilm formation in MRSA (ATCC 33591) while being treated by a combination of and .
Materials And Methods: The minimum inhibitory concentration (MIC) of ethanolic extract of and and also the minimum inhibitory concentration of combination of both extracts were 512, 1024 and 256 μg/ml, respectively; then at concentrations lower than the MIC, expression levels of the desired genes were determined by Real Time PCR.
Results: Based on results, using a combination of two ethanolic extracts had a significant effect on expression of genes involved in biofilm formation in MRSA. The expression level of at 4, 8, 16 h after being treated by herbal extracts of and was 0.293, 0.121, 0.044, respectively. The expression level of was 0.285, 0.097, 0.088, respectively, while that of was 0.087, 0.042, 0.009 at 4, 8 and 16 h, respectively.
Conclusion: This study provided evidence that ethanol extract of and can inhibit the biofilm formation of . As a traditional Iranian medicine, and extracts have a potential antibiofilm formation against MRSA strains.
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