Background And Objectives: The use of plants for the synthesis of nanoparticles has received attention. The present study aimed to evaluate the antibacterial effects of silver nanoparticles synthesized by leaf extract against and .
Materials And Methods: Silver nanoparticles were obtained by reacting silver nitrate solution 2 mM and leaf extract. The AgNPs were characterized by UV-visible spectrophotometer, scanning electron microscopy (SEM), and Fourier transform infrared spectrometer (FTIR). To determine minimum inhibitory concentration and test antibiogram of nanoparticle synthesized, broth micro dilution and agar well diffusion methods were used, respectively.
Results: The zones of bacterial inhibition were 16 ± 0.5 and 9.16 ± 0.28 mm against and using 10 and 0.62 mg/mL AgNPs, respectively. Among the studied bacterial species, silver nanoparticles were more effective on and and less effective on . The highest MIC and MBC of AgNPs (2.5 and 5 mg/mL) were observed for . The lowest MIC and MBC of AgNPs (0.32 and 0.62 mg/mL) were observed for , respectively. The MIC and MBC of AgNPs were found to be 1.25 and 2.5 mg/mL for .
Conclusion: The results clearly indicated that AgNPs have potential antimicrobial activity against Gram-positive and Gram-negative bacteria.
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