Inhibition of prostaglandin biosynthesis leads to suppressed ovarian development in Spodoptera exigua.

Prostaglandins (PGs) are a group of eicosanoids that are C20 oxygenated polyunsaturated fatty acids. PGs can mediate various physiological processes such as immunity, salivary secretion, excretion, and reproduction in insects. The objective of this study was to determine the effect of PG on oocyte development in Spodoptera exigua, a lepidopteran insect known to biosynthesize PGs. Polytrophic ovarioles of S. exigua females exhibited follicle development in germarium, in which oocytes were distinct from nurse cells. During vitellogenesis, nurse cells degenerated by losing cytoplasm called "nurse cell dumping" while oocytes showed increase in cell volume. When PG biosynthesis inhibitors such as ibuprofen or aspirin were applied, nurse cell dumping was not complete and no chorion was formed, thus preventing egg formation. However, addition of PGE significantly rescued such inhibition and resumed oocyte development and choriogenesis. To support the observation with genetic factor, RNA interference (RNAi) specific to peroxynectins (Pxts: Se-Pxt1 and Se-Pxt2) known to act as insect cyclooxygenase was performed to suppress PG biosynthesis. Both Se-Pxt1 and Se-Pxt2 were highly expressed in the ovary of control female. RNAi treatment against Se-Pxt1 or Se-Pxt2 specifically suppressed target genes and inhibited oocyte development. Addition of PGE to adults treated with RNAi rescued the suppressed development of oocytes. Results of this study suggest that PGs can stimulate oocyte development as autocrine/paracrine mediators of vitellogenesis and choriogenesis in insects.