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Correlation between rrs gene mutations and amikacin resistance in Mycobacterium abscessus: implications for fitness cost and clinical prevalence.
J Antimicrob Chemother
January 2025
Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui 230601, China.
Objectives: Amikacin is crucial for treating Mycobacterium abscessus (Mab) infections, with resistance primarily attributed to rrs gene mutations. The correlation between specific mutations and amikacin susceptibility, along with the associated fitness cost, requires further investigation.
Methods: We isolated spontaneous amikacin-resistant mutants in vitro and identified their mutation sites in the rrs gene via Sanger sequencing, which were then compared with existing reports.
A new method for detecting mixed Mycobacterium tuberculosis infection and reconstructing constituent strains provides insights into transmission.
Genome Med
January 2025
Department of Epidemiology of Microbial Disease, Yale School of Public Health, 60 College Street, New Haven, CT, USA.
Background: Mixed infection with multiple strains of the same pathogen in a single host can present clinical and analytical challenges. Whole genome sequence (WGS) data can identify signals of multiple strains in samples, though the precision of previous methods can be improved. Here, we present MixInfect2, a new tool to accurately detect mixed samples from Mycobacterium tuberculosis short-read WGS data.
View Article and Find Full Text PDFA rapid and simple MALDI-TOF MS lipid profiling method for differentiating from .
J Clin Microbiol
January 2025
Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Higashimurayama, Tokyo, Japan.
, a slow-growing nontuberculous mycobacterium, causes Buruli ulcer, a neglected tropical disease. Distinguishing from related species, including , poses challenges with respect to making accurate identifications. In this study, we developed a rapid and simple identification method based on mycobacterial lipid profiles and used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze the lipid profiles of ( = 35) and ( = 19) isolates.
View Article and Find Full Text PDFUnveiling significant regrowth and potential risk of nontuberculous mycobacteria in hospital water supply system.
Water Res
January 2025
Department of Urban Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro, Tokyo 153-8904, Japan. Electronic address:
The health burden of waterborne nontuberculous mycobacteria (NTM) is a rising concern. While the water supply systems can serve as a potential reservoir for NTM, their abundance, diversity, and transmission pathways remain unknown. This study aimed to characterize the prevalence and regrowth of NTM in building water supply system in a hospital where many M.
View Article and Find Full Text PDFCytokine and Chemokine Responses of Peripheral Blood Mononuclear Cells from Dogs Infected with .
Pathogens
December 2024
Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, UK.
Mycobacterial infections are an important emerging zoonosis in companion animals for which diagnostic options remain imperfect, and the canine immunological response to these infections has been poorly investigated. We sought to further define the cellular response of peripheral blood mononuclear cells (PBMCs) from dogs infected with , as determined using a commercial interferon-gamma response assay (IGRA). To this end, PBMCs from healthy or infected dogs were collected.
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