AI Article Synopsis
- The preparation of subproteome fractions helps enrich lesser-represented proteins and gives insights into their cellular locations.
- This text discusses methods for preparing specific protein fractions from Trypanosoma cruzi, focusing on surface, extracellular, and nuclear proteins.
- These protocols are intended for use in further subproteome analysis to better understand the protein landscape of T. cruzi.
Article Abstract
The preparation of subproteome fractions prior to proteome analysis provides both the enrichment of proteins sub-represented in global proteome analysis and information on the cellular localization of the identified proteins. Here we describe protocols for the preparation of Trypanosoma cruzi surface and extracellular and nuclear fractions for further subproteome analysis.
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| http://dx.doi.org/10.1007/978-1-4939-9148-8_6 | DOI Listing |
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