Proteins secreted by skin have great potential as biomarkers for interpreting skin conditions. However, inconvenience in handling and bulky size of existing methods are existing limitations. Here, we describe a thumb-nail sized patch with the array of microdisks which captures multiple proteins from the skin surface. Microdisks with antibody on the surface enable multiplexed immunoassay. By self-assembly, microdisks are placed into 2-dimensional arrays on adhesive tape. The proposed Enzyme-Linked Immunospot array on a Patch shows sufficient sensitivity for IL-1α, IL1RA, IL-17A, IFN-g, and TNF-α, while IL-6 and IL-1β are non-detectable in some cases. As demonstrations, we quantified cytokines from different skin regions and volunteers in a high-spatial-resolution.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404946 | PMC |
| http://dx.doi.org/10.1063/1.5032170 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
ELISpots for Detecting Antigen-Specific Memory B Cells in Infection or Autoimmunity.
Methods Mol Biol
July 2024
Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Newark, NJ, USA.
Enzyme-Linked Immunosorbent Spot assay (ELISpot) is an immunoassay used to quantify individual protein-specific secreting cells. Proteins secreted by cells cultured in ELISpot plates (96- or 8-well format) bind to a specific antigen bound to a PVDF membrane at the bottom of the well. A detection antibody followed by an enzymatic reaction is used to identify secreted protein bound to the membrane coated antigen.
View Article and Find Full Text PDFUnbiased, High-Throughput Identification of T Cell Epitopes by ELISPOT.
Methods Mol Biol
June 2023
Research & Development Department, Cellular Technology Limited, Shaker Heights, OH, USA.
Recent systematic immune monitoring efforts suggest that, in humans, epitope recognition by T cells is far more complex than has been assumed based on minimalistic murine models. The increased complexity is due to the higher number of HLA loci in humans, the typical heterozygosity for these loci in the outbred population, and the high number of peptides that each HLA restriction element can bind with an affinity that suffices for antigen presentation. The sizable array of potential epitopes on any given antigen is due to each individual's unique HLA allele makeup.
View Article and Find Full Text PDFImmunodominant MHC-II (Major Histocompatibility Complex II) Restricted Epitopes in Human Apolipoprotein B.
Circ Res
July 2022
Center for Autoimmune Disease, Laboratory of Inflammation Biology (P.R., S.S.A.S., M.B., J.V., V.S., M.O., J.M., K.L.), La Jolla Institute for Immunology, CA.
Background: CD (cluster of differentiation) 4 T-cell responses to APOB (apolipoprotein B) are well characterized in atherosclerotic mice and detectable in humans. CD4 T cells recognize antigenic peptides displayed on highly polymorphic HLA (human leukocyte antigen)-II. Immunogenicity of individual APOB peptides is largely unknown in humans.
View Article and Find Full Text PDFMeasuring single-cell protein secretion in immunology: Technologies, advances, and applications.
Eur J Immunol
June 2021
ETH Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, D-CHAB, ETH Zürich, Zürich, Switzerland.
The dynamics, nature, strength, and ultimately protective capabilities of an active immune response are determined by the extracellular constitution and concentration of various soluble factors. Generated effector cells secrete such mediators, including antibodies, chemo- and cytokines to achieve functionality. These secreted factors organize the individual immune cells into functional tissues, initiate, orchestrate, and regulate the immune response.
View Article and Find Full Text PDFHuman Antibody Responses Following Vaccinia Immunization Using Protein Microarrays and Correlation With Cell-Mediated Immunity and Antibody-Dependent Cellular Cytotoxicity Responses.
J Infect Dis
October 2021
Emmes, Rockville, Maryland, USA.
Article Synopsis
- The study investigates the immunological responses to the modified vaccinia Ankara (MVA) smallpox vaccine using 523 participants who received either lyophilized or liquid MVA in different administration methods.
- Results showed that MVA triggered significant antibody production against several viral proteins, particularly after 28 and 42 days, with strong correlations between some antibody responses and plaque reduction neutralization tests (PRNT).
- The findings suggest that specific proteins from the MVA vaccine could be potential indicators of protection and may inform future vaccine development strategies.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!