Chemical Composition and in vivo Efficacy of the Essential Oil of Mentha piperita L. in the Suppression of Crown Gall Disease on Tomato Plants.

J Oleo Sci

Research Laboratory of Environmental Sciences and Technologies (LR16ES09), Higher Institute of Environmental Sciences and Technology, University of Carthage.

Published: May 2019

AI Article Synopsis

  • This study explored the antibacterial effects of peppermint essential oil (EO) against the bacteria Agrobacterium tumefaciens, using both lab (in vitro) and plant (in vivo) experiments.
  • The chemical analysis revealed that the EO consists mainly of menthol (33.59%) and iso-menthone (33.00%), with significant bactericidal activity noted across various strains of Agrobacterium, showing minimum inhibitory concentrations (MIC) between 0.01 and 12.50 mg/mL.
  • In tests involving tomato plants, the peppermint EO successfully prevented tumor formation caused by the bacterium at a concentration of 200 mg/mL, indicating its potential as a natural method for controlling bacterial diseases in

Article Abstract

This study was undertaken to determine the antibacterial efficacy of the essential oil (EO) of peppermint (Mentha piperita L.), in vitro and in vivo, against the phytopathogenic bacteria Agrobacterium tumefaciens (A. tumefaciens). The EO composition of M. piperita L. was investigated by Gas chromatography-mass spectrometry (GC/MS) with 26 identified volatile constituents. The major constituents were menthol (33.59%) and iso-menthone (33.00%). This EO exerted a bactericidal activity against multiple strains of Agrobacterium species with minimum inhibitory concentration (MIC) values ranged from 0.01 to 12.50 mg/mL. In planta experiments, M. piperita EO, tested at concentration of 200 mg/mL, completely inhibited the formation of tumors on tomato plants inoculated with pathogenic strain A. tumefaciens ATCC 23308. These results suggest that M. piperita EO could be used to control plant bacterial disease caused by A. tumefaciens.

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Source
http://dx.doi.org/10.5650/jos.ess18261DOI Listing

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