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Distinct molecular properties and functions of small EV subpopulations isolated from human umbilical cord MSCs using tangential flow filtration combined with size exclusion chromatography.
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State Key Laboratory of Reproductive Medicine and offspring health, Nanjing Medical University, Nanjing, China.
As functional derivatives of mesenchymal stem cells (MSCs), small extracellular vesicles (sEVs) have garnered significant attention and application in regenerative medicine. However, the technical limitations for large-scale isolation of sEVs and their heterogeneous nature have added complexity to their applications. It remains unclear if the heterogeneous sEVs represent different aspects of MSCs functions.
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January 2025
Institute for Global Health Innovations, Duy Tan University, Da Nang 550000, Viet Nam; Faculty of Medicine, Duy Tan University, Da Nang 550000, Viet Nam. Electronic address:
The discovery of food-derived biopeptides is becoming increasingly prevalent in the scientific community. Some peptides possess multiple biological functions that can confer health benefits through various mechanisms following ingestion. The present review targets food-derived antioxidant and mineral-binding peptides (AMBPs) including their production procedure i.
View Article and Find Full Text PDFImaged capillary isoelectric focusing and online mass spectrometry for milk whey protein characterization in dairy products.
Anal Biochem
January 2025
Advanced Electrophoresis Solutions Ltd., 380 Jamieson Parkway, Unit 7 and 8, ON, N3C 4N4 Canada; AES Biotech Jiaxing Ltd., No. 501 South Changsheng Road, Economic and Technological Development Zone, Jiaxing City, Zhejiang Province, P.R. China. Electronic address:
Characterizing major bovine milk proteins, including whey and casein, is of significant interest in the dairy industry. The diverse array of protein proteoforms can be different in terms of genetic variation, breed ways, lactation stage, and animal nutritional status. Current routine methods for bovine milk protein profiling are typically based on immunological techniques, infrared spectroscopy, slab gel isoelectric focusing, capillary electrophoresis, and high-performance liquid chromatography.
View Article and Find Full Text PDFIonic liquid coating for stir bar sorptive extraction and its application for extraction and nontargeted screening analysis via TD-GC-Orbitrap-HRMS of pollutants in river water.
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December 2024
Univ Rouen Normandie, FR3038, SMS, UR 3233, F-76000 Rouen, France. Electronic address:
In this study, a novel imidazolium-based ionic liquid (IL) coating was developed for stir bar sorptive extraction (SBSE) using a sol-gel method. The effects of different counterions, conditioning temperatures and polymer compositions were investigated. The stir bar with bis((trifluoromethyl)sulfonyl) amide 1-butyl-3-(3-(triethoxysilyl)propyl)-1H-imidazol-3-ium showed good mechanical and thermal stability with high resistance to water solubilization.
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