Split-QF System for Fine-Tuned Transgene Expression in .

Genetics

Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, M13 9PL, United Kingdom.

Published: May 2019

The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in , is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in , and in other organisms.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499530PMC
http://dx.doi.org/10.1534/genetics.119.302034DOI Listing

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