AI Article Synopsis
- A cDNA library was created using a viral RNA mixture from various garlic viruses, leading to the identification of different viral genomic sequences.
- One of the identified viruses was categorized as garlic virus A (GarV-A), a new virus previously discovered in Japan, and its coat protein was successfully expressed in E. coli for antibody production.
- The generated antibodies specifically recognized GarV-A and related garlic mite-borne viruses, demonstrating their utility in detecting the virus across most garlic cultivars in Argentina using various immunological assays.
Article Abstract
A cDNA library representing the genomes of several garlic viruses was generated using a viral RNA mixture as template. Analysis of randomly selected clones allowed the identification of different viral genomic sequences. On the basis of amino acid and nucleotide sequence comparisons, one of them was assigned to garlic virus A (GarV-A), a novel flexuous, rod-shaped virus recently reported in Japan. The coat protein (CP) of this virus was expressed in Escherichia coli cells and used as immunogen to produce polyclonal antibodies. The expression protein was recognized by an antiserum detecting garlic mite-borne filamentous virus, but did not react with antibodies specific for other garlic viruses. Antibodies raised against the viral CP reacted with extracts infected with garlic mite-borne viruses and fail to recognize preparations of onion yellow dwarf potyvirus, leek yellow stripe potyvirus, and carnation latent carlavirus. The same antibodies decorated viral particles exhibiting a modal length of 586 nm in immuno electron microscopy with decoration assays. Double-antibody enzyme-linked immunosorbent assays and tissue printing assays performed with these antibodies allowed detection of GarV-A in most garlic cultivars used in Argentina.
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| http://dx.doi.org/10.1094/PDIS.1997.81.9.1005 | DOI Listing |
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