Electron holography in Fraunhofer region was realized by using an asymmetric double slit. A Fraunhofer diffraction wave from a wider slit worked as an objective wave interfered with a plane wave from a narrower slit as a reference wave under the pre-Fraunhofer condition and recorded as a hologram. Here, the pre-Fraunhofer condition means that the following conditions are simultaneously satisfied: single-slit observations are performed under the Fraunhofer condition and the double-slit observations are performed under the Fresnel condition. Amplitude and phase distributions of the Fraunhofer diffraction wave were reconstructed from the hologram by the Fourier transform reconstruction method. The reconstructed amplitude and phase images corresponded to Fraunhofer diffraction patterns; in particular, the phase steps of π at each band pattern in the phase image were confirmed. We hope that the developed Fraunhofer electron holography can be extended to a direct phase detection method in the reciprocal space.
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http://dx.doi.org/10.1093/jmicro/dfz007 | DOI Listing |
Nanophotonics
August 2024
Institute of Solid State Physics, Friedrich Schiller University Jena, Max-Wien-Platz 1, 07743 Jena, Germany.
Gallium phosphide (GaP) offers unique opportunities for nonlinear and quantum nanophotonics due to its wide optical transparency range, high second-order nonlinear susceptibility, and the possibility to tailor the nonlinear response by a suitable choice of crystal orientation. However, the availability of single crystalline thin films of GaP on low index substrates, as typically required for nonlinear dielectric metasurfaces, is limited. Here we designed and experimentally realized monolithic GaP metasurfaces for enhanced and tailored second harmonic generation (SHG).
View Article and Find Full Text PDFCombining the propagation and geometric phases in a metasurface facilitates the independent control of multiple parameters of the light field. However, the geometric phase often displays a random distribution, making it difficult to observe directly. We introduce a frequency-dependent phase response: at frequency f, there is a superposition of the geometric and propagation phases, whereas at frequency f, the propagation phase remains constant, and only the geometric phase is applied.
View Article and Find Full Text PDFMethods Enzymol
November 2024
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany; Translational Neuroinflammation and Automated Microscopy TNM, Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Göttingen, Germany; Department of Neurology, University Medical Center Göttingen, Göttingen, Germany. Electronic address:
The mitochondrial translocases of the outer membrane (TOM) and of the inner membrane (TIM) act together to facilitate the import of nuclear-encoded proteins across the mitochondrial membranes. Stimulated Emission Depletion (STED) super-resolution microscopy enables the in situ imaging of such complexes in single cells at sub-diffraction resolution. STED microscopy requires only conventional sample preparation techniques and provides super-resolved raw data without the need for further image processing.
View Article and Find Full Text PDFSci Rep
September 2024
Autonomous Manufacturing & Process R&D Department, Korea Institute of Industrial Technology (KITECH), Sangnok-gu, Ansan-si, 15588, Korea.
Inkjet printers are key technologies in manufacturing organic light-emitting diodes and quantum dot light-emitting diode panels, but precise measurement and control of inkjet droplets remains challenging. The international standard, IEC 62899-302-1, uses shadow image-based measurement with high magnification microscopes to observe picoliter-sized droplets. However, high magnification lens results in a shallow depth of field or narrow optimal measurement area, causing the blurring image if the droplet does not pass through the optimal measurement area.
View Article and Find Full Text PDFFront Cell Dev Biol
August 2024
Physics Department, Sofia University "St. Kliment Ohridski", Sofia, Bulgaria.
Bone substitutes are widely employed for applications in orthopedic surgery for the replacement of injured bone. Among the diverse methods that are used to design 3D bioceramic matrices, Freeze Foaming has gained attention, since it provides the ability to tune the shape of the created structures. One of the major problems related to these constructs is the lack of porosity at the outwards sides (holder) of the scaffold, thus reducing the cellular affinity and creating a rejection of the implant.
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