Evaluation of clinical utility of P53 gene variations in repeated implantation failure.

Repeated implantation failure (RIF) is one the most common causes which showed during IVF (In vitro fertilization) procedure. We aim to evaluate the possibility role of nucleotide changes in rs1042522 (R72P; G/C) and rs17878362 (Ins16bp; N/D) of P53 gene in patients with RIF. In a case-control survey, we have considered 200 women, consisting of 100 cases with RIF and 100 women with the normal pregnancy. In order to determine the genotype frequencies, we used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The NN + ND/DD (dominant) genotype frequencies of rs17878362 variant revealed a significant difference between two groups (P = 0.001; OR 2.652; 95% CI 1.480-4.754). In addition, the CC + GC/GG (recessive) genotype frequencies of rs1042522 (R72P) variant indicated a significant difference between two groups (P = 0.018; OR 3.353; 95% CI 1.169-9.616). Our findings suggested that rs1042522 (R72P; G/C) and rs17878362 (Ins16bp; N/D) of P53 gene polymorphisms could be a genetic predisposing factor for RIF.