Introduction: Filariasis, a neglected tropical helminth disease needs vaccine besides mass drug administration for its successful eradication.
Methods: An attempt was made to produce a fusion protein (P-TUFT-ALT-2) of abundant larval transcript protein-2 and Tuftsin to enhance its immunogenicity. The fusion construct was expressed in , a nonexpensive commercial expression system. This study focused on the evaluation of immunological response produced by P-TUFT-ALT-2 in Balb/c mice.
Result And Discussion: P-TUFT-ALT-2 showed an enhanced IgG peak titre compared to expressed E-ALT-2 and expressed P-ALT-2. IgG2b, IgG2a and IgG1 production were predominant indicating a balanced Th1/Th2 response. P-TUFT-ALT-2 also induced about 28% and 9.5% higher splenocyte proliferation over control and E-ALT-2 respectively. Splenocytes produced predominant IFN-γ followed by IL-5, IL-2 and IL-10 specifying a balanced Th1/Th2 response. P-TUFT-ALT-2 showed 55% to 80% with an average of 65% cytotoxicity in L3 larvae in ADCC assay.
Conclusion: This experiment validates P-TUFT-ALT-2 as a potential vaccine candidate for human lymphatic filariasis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378782 | PMC |
| http://dx.doi.org/10.1016/j.parepi.2019.e00092 | DOI Listing |
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