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Validation of a new extraction-free real-time PCR test to detect MPOX virus.
Diagn Microbiol Infect Dis
June 2024
Esoteric Testing/R&D Laboratory, Tampa General Hospital, 1 Tampa General Circle, Tampa, FL 33606, USA.
Article Synopsis
- * The study assessed the BioGX Xfree hMPXV/OPXV test's ability to detect Mpox virus DNA in human samples using two different PCR platforms.
- * Results showed that the Xfree hMPXV/OPXV test is sensitive and specific, with the pixl.16 platform offering benefits like a smaller size and quicker turnaround time due to its extraction-free process.
Clinical evaluation of the molecular-based BD SARS-CoV-2/Flu for the BD MAX™ system.
J Clin Virol
October 2021
Tricore Reference Laboratory, 1001 Woodward Place, N.E., Albuquerque, NM 87102, USA. Electronic address:
Background: COVID-19 and influenza (flu) share similar clinical symptoms. Therefore, differential detection of these viruses during the respiratory virus season will be an important component for proper patient triage, management, and treatment.
Objectives: Establish the diagnostic performance related to SARS-CoV-2 and Flu A/B detection for the BD SARS-CoV-2/Flu for BD MAX™ System ("MAX SARS-CoV-2/Flu") multiplex assay.
Evaluation of the BioGX BD-Max PCR assay for detection of pathogenic Bordetella.
Pathology
April 2019
Centre for Infectious Diseases and Microbiology - Public Health, Westmead Hospital, NSW, Australia; Sydney Medical School, The University of Sydney, NSW, Australia; Centre for Infectious Diseases and Microbiology Laboratory Services, NSW Health Pathology, Sydney, NSW, Australia.
Evaluation of the Seegene Allplex™ Respiratory Panel for diagnosis of acute respiratory tract infections.
Acta Clin Belg
December 2019
Department of Laboratory Medicine, Medical Microbiology, Ghent University Hospital, Ghent , Belgium.
The Seegene Allplex Respiratory panel was retrospectively challenged using a collection of quality control samples (QCMD) and clinical samples previously analysed with validated routine methods. A collection of 111 samples [43 QCMD samples, 13 bronchoalveolar lavage fluids and 55 nasopharyngeal aspirates/swabs] was tested with Seegene Allplex. The clinical samples were tested previously using either FTD® Respiratory Pathogens 21 qPCR assay (Fast Track Diagnostics), an in-house multiplex PCR for , or BioGX Sample-Ready Atypical pneumo panel (Becton Dickinson).
View Article and Find Full Text PDFEvaluation of the GeneXpert for Human Monkeypox Diagnosis.
Am J Trop Med Hyg
February 2017
Poxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Enteric and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia.
Monkeypox virus (MPXV), a zoonotic orthopoxvirus (OPX), is endemic in the Democratic Republic of Congo (DRC). Currently, diagnostic assays for human monkeypox (MPX) focus on real-time quantitative polymerase chain reaction (PCR) assays, which are typically performed in sophisticated laboratory settings. Herein, we evaluated the accuracy and utility of a multiplex MPX assay using the GeneXpert platform, a portable rapid diagnostic device that may serve as a point-of-care test to diagnose infections in endemic areas.
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