Between March and May 1983, tongues and esophagi of 355 adult ewes from Colorado and Idaho were examined for grossly visible sarcocysts. Sarcocysts of Sarcocystis gigantea were found in 35 sheep. Cats fed sarcocysts from these naturally infected sheep shed sporocysts in their feces. Two adult ewes and 12 lambs inoculated with 1,000 to 1,000,000 sporocysts were euthanatized at postinoculation days (PID) 146, 230, 265, 391, 721, and 882, and their tissues were fed to Sarcocystis-free cats. All inoculated sheep remained clinically normal except for mild pyrexia between PID 12 and 18. Sarcocysts first became grossly visible at PID 391 and sarcocysts from sheep first became infectious for cats at PID 230.
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Parasitol Res
September 2024
National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People's Republic of China.
Sarcocystis infection in sheep has caused significant economic losses in the livestock industry, and the genetic similarity among Sarcocystis species highlights the need for precise diagnostic methods in sheep. This study developed a loop-mediated isothermal amplification (LAMP) method targeting COX-1 and 28S rRNA genes to detect Sarcocystis tenella and Sarcocystis gigantea, respectively. The LAMP method exhibited high specificity, selectively amplifying target DNA sequences without cross-reactivity with closely related protozoa, such as Toxoplasma gondii and Neospora caninum.
View Article and Find Full Text PDFInt J Parasitol Parasites Wildl
August 2024
Department of Veterinary Medical Sciences (DIMEVET), Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Emilia, BO, Italy.
Synanthropic rodents play a crucial role in maintaining the life cycle of in anthropized regions and can serve as indicators of environmental oocyst contamination. This investigation aimed to explore the occurrence of infection within synanthropic rodent populations using a molecular diagnostic technique targeting the 18S rDNA gene, which is generic for Coccidia, with subsequent specific PCR confirmation. We examined 97 brown rats (), 67 black rats (), 47 house mice (), and 1 common shrew ().
View Article and Find Full Text PDFVet Sci
February 2024
Department of Animal Pathology, Faculty of Veterinary Sciences, Universidad de Zaragoza, 50013 Zaragoza, Spain.
spp. are complex apicomplexan parasites that cause a substantial economic impact on livestock used for meat production. These parasites are present worldwide.
View Article and Find Full Text PDFFront Vet Sci
September 2023
Department II of Anatomy, Faculty of Medicine, University Hospital Cologne, Cologne, Germany.
Introduction: is a genus of cyst-forming parasites that infest both humans and livestock. Some parasites cause clinical and subclinical diseases in their hosts, resulting in economic losses.
Methods: Esophagus, diaphragm, and skeletal muscle from slaughtered sheep and goats were examined macroscopically, microscopically, and ultrastructurally and subjected to DNA analysis.
Vet Res Forum
August 2023
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Duhok University, Duhok, Iraq.
This study aimed to identify species isolated from macroscopic sarcocysts of naturally infected sheep and goats using histopathological and molecular studies. A total of 260 macrosarcocyst samples were randomly collected from 1,337 infected sheep and goats slaughtered at different abattoirs in Duhok province, Iraq, from May 2021 to June 2022. The macroscopic cysts, which were found in the esophagus, diaphragm, and abdominal muscles, were classified into fat and thin cysts.
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