AI Article Synopsis
- Understanding how cells interact with their environment is crucial for biological research, but observing these interactions in real-time is difficult.
- A new method has been developed that uses engineered DNA motifs and synthetic cofactors to create DNA-based sensors for cell surfaces.
- This approach has successfully demonstrated real-time imaging of key signaling molecules being expelled from cells, showcasing its versatility and effectiveness.
Article Abstract
The comprehensive understanding of the mechanisms underlying the interaction of cells with their membrane microenvironment is of great value for fundamental biological research; however, tracking biomolecules on cell surfaces with high temporal and spatial resolution remains a challenge. Herein, a modular strategy is presented for the construction of cell surface DNA-based sensors by engineering DNA motifs and synthetic cofactors. In this strategy, a stimuli-reactive organic molecule is employed as the cofactor for the DNA motif, and the self-assembly of them forms a FRET-based holo DNA-based sensor. With the use of the DNA-based sensors, the versatility of this modular strategy has been demonstrated in the ratiometric imaging of the cellular extrusion process of endogenous signaling molecules, including sulfur dioxide derivatives and nitric oxide.
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| http://dx.doi.org/10.1002/anie.201901320 | DOI Listing |
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