Quantitative co-localization analysis, combined with other in vivo and in vitro techniques, can provide valuable information about the interaction and cooperative function of two proteins. Here we describe in detail the technique of quantitative co-localization analysis of two enamel matrix proteins, amelogenin and ameloblastin, in developing mouse enamel.
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LAP-MALDI MS analysis of amelogenin from teeth for biological sex estimation.
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Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6DX, United Kingdom. Electronic address:
The biological sex estimation of human individuals can be achieved by extracting fragments of the amelogenin protein from small areas of tooth enamel. The amelogenin gene can be found on both sex chromosomes (X and Y) with chromosome-specific differences in its sequence, and consequently the sequences of the expressed protein in teeth. Virtually all current analytical techniques used to identify the occurrence of the male Y chromosome-specific proteoform employ proteoform-specific peptide analysis by LC-ESI MS/MS, which typically results in longer analytical times due to the LC separation step, despite recent efforts of shortening the LC step.
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iScience
October 2024
Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China.
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Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA 90033, USA; Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90033, USA. Electronic address:
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Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA 90033, USA. Electronic address:
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