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Biomass grown in wastewater treatment photobioreactors is a cheap raw material with high contents of carbohydrates, proteins and lipids. This work studies the production of fermentable monosaccharides from three biomasses grown in piggery wastewater (P), domestic wastewater (W) and synthetic medium (S) by applying chemical pretreatment and enzymatic hydrolysis, using a Taguchi design. ANOVA identified temperature, chemical reagent type and chemical reagent concentration as significant operational parameters. However, the biomass concentration, pretreatment time, enzyme dosage and enzymatic hydrolysis time had no remarkable effect. The bacterial content of the biomass had no relevant impact on carbohydrate and protein solubilisation but had a remarkable effect on the degradation of the released carbohydrates (57, 60 and 37% for P, W and S), while also affecting lipid solubilisation. Pretreatment with HCl 2 M at 120 °C resulted the optimal conditions, achieving a monosaccharide recovery of 53, 59 and 80% for P, W and S biomasses, respectively.
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http://dx.doi.org/10.1016/j.biortech.2019.02.082 | DOI Listing |
AMB Express
December 2024
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, El Cerrillo Piedras Blancas, 50295, Toluca, Estado de México, Mexico.
Reducing greenhouse gas (GHG) emissions from livestock is a crucial step towards mitigating the impact of climate change and improving environmental sustainability in agriculture. This study aimed to evaluate the effects of Yucca schidigera extract, chitosan, and chitosan nanoparticles as feed additives on in vitro GHG emissions and fermentation profiles in ruminal fluid from bulls. Total gas, CH, CO, and HS emissions (up to 48 h), rumen fermentation profiles, and CH conversion efficiency were measured using standard protocols.
View Article and Find Full Text PDFJ Pharm Biomed Anal
December 2024
Guang'an Men Hospital, China Academy of Chinese Medical Sciences, Beijing 100010, China. Electronic address:
Daidzin, as one of isoflavone glycosides, has been reported to have multiple activities with few absorbed into body. However, the metabolic behavior of daidzin by intestinal flora has not been researched, that this defect severely constrains its applications. In this study, daidzin and its metabolites were qualitatively and quantitatively analyzed by HPLC and ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) in the fermentation system for daidzin and fecal bacteria.
View Article and Find Full Text PDFInt J Med Mushrooms
December 2024
School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408100, P.R. China.
Species of the genus Ganoderma are important medicinal and edible mushrooms, with polysaccharides being its main active ingredient. The submerged fermentation of Ganoderma is an important source of polysaccharides in the mycelia. In this study, a single factor test and an orthogonal test were employed to optimize the medium and fermentation conditions with the objective of obtaining a high content of polysaccharide in the liquid submerged fermentation culture mycelia of Ganoderma species.
View Article and Find Full Text PDFHeliyon
December 2024
Research Laboratory of Environmental Sciences and Sustainable Development, LR18ES32, University of Sfax, Tunisia.
The annotated and predicted genomes of five archaeal strains (AS1, AS2, AS8, AS11 and AS19), isolated from Sfax solar saltern sediments (Tunisia) and affiliated with , were performed by RAST webserver (Rapid Annotation using Subsystem Technology) and NCBI prokaryotic genome annotation pipeline (PGAP). The results showed the ability of strains to use a reduced semi-phosphorylative Entner-Doudoroff pathway for glucose degradation and an Embden-Meyerhof one for gluconeogenesis. They could use glucose, fructose, glycerol, and acetate as sole source of carbon and energy.
View Article and Find Full Text PDFFood Chem X
December 2024
College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, Jilin, PR China.
() residues polysaccharide (FVRP) is a high molecular weight polysaccharide with diverse bioactivities extracted from residues (FVR). However, high molecular weight polysaccharides have been shown to face significant challenges in crossing the cell membrane barrier, thereby limiting their absorption and application in the body. Therefore, an ultrasonic-assisted HO-Fe method was employed for the first time to degrade FVRP, resulting in the production of a new polysaccharide, FVRPF.
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