AI Article Synopsis
- Exosomes are tiny vesicles released by various cell types, playing a key role in cell communication and potential clinical applications like non-invasive disease diagnosis.
- Recent research highlights ultracentrifugation as a straightforward and effective method for purifying exosomes from cell culture supernatants.
- The chapter outlines a detailed procedure for isolating exosomes and characterizing them based on size, quantity, and protein expression using techniques such as electron microscopy and western blotting.
Article Abstract
Exosomes are small vesicles of endosomal origin secreted by most cell types. Recent studies have identified exosomes as important mediators of intercellular communication and as important source materials for many clinical applications, including minimal invasive disease diagnosis. Exosomes have been purified from in vitro cell culture supernatants by many different methods; however the most simple and reliable method involves purification by ultracentrifugation. This chapter describes a detailed protocol for isolating exosomes from cell culture-conditioned medium using ultracentrifugation and their characterization based upon size, number, and protein expression by several complementary methods such as transmission electron microscopy, nanoparticle tracking analysis, western blotting, and flow cytometry.
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| http://dx.doi.org/10.1007/978-1-4939-9133-4_19 | DOI Listing |
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