AI Article Synopsis
- Particle exclusion assays help visualize the presence of hyaluronan around cells, which can indicate the cellular state and differ between cell types.
- The method utilizes aggrecan, a hyaluronan-binding proteoglycan, to enhance and stabilize the surrounding matrix for better detection.
- Microscopic analysis allows researchers to quantify the hyaluronan matrix by observing clear zones around cells where added red blood cell particles are excluded.
Article Abstract
Particle exclusion assays are used to visualize pericellular envelopes with a high content of hyaluronan. Pericellular hyaluronan is basally abundant in certain cell types while in others it is deposited in a highly dynamic manner in response to specific conditions and its presence may indicate cellular status. This assay, described here, is a quick semiquantitative approach to detecting pericellular hyaluronan using the hyaluronan-binding proteoglycan, aggrecan, to stabilize and amplify the surface matrix. Hyaluronan matrix can then be observed and quantified by microscopic image analysis of clear zones around individual cells, from which exogenously added fixed red blood cell particles are excluded.
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| http://dx.doi.org/10.1007/978-1-4939-9133-4_9 | DOI Listing |
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