AI Article Synopsis
- The study aimed to use recombinant E. coli with mouse arachidonate 8S-lipoxygenase (8S-LOX) to produce hydroxylated forms of polyunsaturated fatty acids like arachidonic acid (AA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA).
- The optimal conditions for hydroxylation were determined to be pH 7.5, 30 °C, 5% ethanol, and a specific amount of cells and substrate, leading to significant hydroxylation yields for the respective fatty acids over set time periods.
- This research marks the first successful biotechnological synthesis of specific hydroxylated fatty acids (8
Article Abstract
Objective: To quantitatively hydroxylate 8S- and 10S-positions on polyunsaturated fatty acids by recombinant Escherichia coli cells expressing mouse arachidonate 8S-lipoxygenase (8S-LOX).
Results: Hydroxylated products gained from the conversion of arachidonic acid (20:4, AA), eicosapentanoic acid (20:5, EPA), and (22:6, DHA) by recombinant E. coli cells containing 8S-LOX from mouse were identified as 8S-hydroxy-5,9,11,14(Z,E,Z,Z)-eicosatetranoic acid (8S-HETE), 8S-hydroxy-5,9,11,14,17(Z,E,Z,Z,Z)-eicosapentanoic acid (8S-HEPE), and 10S-hydroxy-4,8,12,14,16,19(Z,E,Z,Z,Z,Z)-docosahexaenoic acid (10S-HDoHE), respectively. Under the optimal hydroxylation conditions of pH 7.5, 30 °C, 5% (v/v) ethanol, 15 g cells l, and 5 mM substrate, AA, EPA, and DHA were hydroxylated into 4.37 mM 8S-HETE, 3.77 mM 8S-HEPE, and 3.13 mM 10S-HDoHE for 60, 90, and 60 min, with 87, 75, and 63% molar conversions, respectively.
Conclusion: To the best of our knowledge, this is the first quantitatively biotechnological production of 8S-HETE, 8S-HEPE, and 10S-HDoHE.
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| http://dx.doi.org/10.1007/s10529-019-02659-5 | DOI Listing |
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