Autophagy-mediated degradation exhibits promotion of PHLPP1 protein translation.

PHLPP1 (PH domain and leucine rich repeat protein phosphatase 1) is a newly identified family of Ser/Thr phosphatases that catalyzes the dephosphorylation of a conserved regulatory motif of the AGC kinases resulting in a tumor suppressive function, while CDKN1B/p27 also acts as a tumor suppressor by regulating cell cycle, senescence, apoptosis, and cell motility. Our most recent studies reveal that CDKN1B is required for PHLPP1 abundance, which contributes to the inhibition of carcinogenic arsenite-induced cell malignant transformation through inhibition of RPS6-mediated translation. However, nothing is known about the mechanisms underlying the crosstalk between these 2 key tumor suppressors in intact cells. Here, for the first time to the best of our knowledge, we show that CDKN1B is able to promote PHLPP1 protein translation by attenuating the abundance of , which binds directly to the 5'untranslated region (UTR) of mRNA. Further studies indicate that the attenuation of expression is due to macroautophagy/autophagy-mediated degradation of in an SQSTM1/p62-dependent fashion. Moreover, we have determined that is upregulated by CDKN1B at the level of transcription enhancing SP1 protein stability in an HSP90-depdendent manner. Collectively, our studies prove that: 1) SQSTM1 is a CDKN1B downstream effector responsible for CDKN1B-mediated autophagy; 2) by promoting the autophagy-mediated degradation of , CDKN1B exerts a positive regulatory effect on PHLPP1 translation; 3) suppresses PHLPP1 translation by binding directly to its mRNA 5'-UTR, rather than classical binding to the 3'-UTR. These findings provide significant insight into understanding the crosstalk between CDKN1B and PHLPP1. ATG: autophagy related; ACTB: actin beta; BAF: bafilomycin; BECN1: beclin 1; /: cyclin-dependent kinase inhibitor 1B; CHX: cycloheximide; DMEM: dulbecco's modified eagle medium; FBS: fetal bovine serum; GAPDH: glyceraldehyde -3-phosphate dehydrogenase; : hypoxia inducible factor 1, alpha subunit; : heat shock protein 90; JUN: Jun proto-oncogene, AP1 transcription factor subunit; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MG132: proteasome inhibitor; : mechanistic target of rapamycin kinase; : PH domain and leucine rich repeat protein phosphatase 1; : PH domain and leucine rich repeat protein phosphatase 2; : protein phosphatase 2 C; RPS6: ribosomal protein S6; : trans-acting transcription factor 1; : sequestosome 1; TUBA: alpha tubulin; 3'-UTR; 3'-untranslated region; 5'-UTR: 5'-untranslated region.