AI Article Synopsis
- The Cre/loxP system is essential in neuroscience for targeting specific cell types, especially late-born glial cells that produce myelin.
- Researchers have access to many conditional mutants and Cre-expressing mouse lines, enabling effective studies of oligodendrocytes and their precursors in living organisms.
- This chapter outlines methods for targeting myelinating glial cells in mice for research purposes and highlights key Cre driver lines used in this developing area of study.
Article Abstract
Cell-type-specific gene targeting with the Cre/loxP system has become an indispensable technique in experimental neuroscience, particularly for the study of late-born glial cells that make myelin. A plethora of conditional mutants and Cre-expressing mouse lines is now available to the research community that allows laboratories to readily engage in in vivo analyses of oligodendrocytes and their precursor cells. This chapter summarizes concepts and strategies in targeting myelinating glial cells in mice for mutagenesis or imaging, and provides an overview of the most important Cre driver lines successfully used in this rapidly growing field.
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| http://dx.doi.org/10.1007/978-1-4939-9072-6_15 | DOI Listing |
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