AI Article Synopsis
- Primary cultures of rodent brain cells are important for researching neurobiology at the molecular and cellular levels.
- The chapter outlines techniques for purifying and culturing oligodendroglial cells from mouse brains during the perinatal period.
- It also explains how to co-culture these oligodendrocytes with neurons to study their growth, maturity, and role in myelination.
Article Abstract
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.
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| http://dx.doi.org/10.1007/978-1-4939-9072-6_5 | DOI Listing |
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