Gastrointestinal (GI) microflora is an important system in the host, as it has both pathogenic and probiotic bacteria. Most of the studies were focused on the human gut microflora and the available information on the intestinal microflora of goats was limited. This urged the need to inspect the impacts of the goat's gut microflora. Metagenomic investigation of probiotic bacteria in the GI tract of goat is one of the challenging streams because of the less available data of the uncultivable bacteria. In our report, comparative analysis of metagenomic and enrichment samples of goat intestinal content was done and this approach will be helpful in analyzing the identification of uncultivable and cultivable probiotic bacteria. This study mainly focused on three key probiotic adhesion genes, such as EF-Tu, mapA, and mub. The GI of four different goats were investigated for these genes. The data from this study showed that there is a wide diversity of these genes among goat intestinal samples.
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http://dx.doi.org/10.1002/jcb.28508 | DOI Listing |
Int J Biol Macromol
January 2025
Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (Bangkhunthian Campus), Bangkok 10150, Thailand. Electronic address:
This study aimed to produce a novel resistant maltodextrin (RMD) from the remaining starch in cassava pulp via pyrodextrinization and enzymatic hydrolysis. The optimum conditions involved a temperature of 180 °C, 0.5 % HCl, and a reaction time of 5 h, resulting in a significant RMD yield (18.
View Article and Find Full Text PDFGut Microbes
December 2025
Gastroenterology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.
Irritable bowel syndrome (IBS) is a multifactorial condition with heterogeneous pathophysiology, including intestinal permeability alterations. The aim of the present study was to assess the ability of a probiotic blend (PB) consisting of two strains (CECT7484 and CECT7485) and one strain of (CECT7483) to recover the permeability increase induced by mediators from IBS mucosal biopsies and to highlight the underlying molecular mechanisms. Twenty-one IBS patients diagnosed according to ROME IV criteria (11 IBS-D and 10 IBS-M) and 7 healthy controls were enrolled.
View Article and Find Full Text PDFFood Sci Nutr
January 2025
Food and Nutrition Policy and Planning Research Department, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute Shahid Beheshti University of Medical Sciences Tehran Iran.
This study aimed to estimate the quantity and trends of salt intake from industrial fermented dairy products, develop strategies to reduce salt content, and inform policymakers on promoting public health through healthier dairy options. A cross-sectional study was conducted on fermented dairy products. Seventy-nine random samples were selected, and the salt (NaCl %) content was determined by potentiometric titration after sample preparation and homogenization; also samples were analyzed for their moisture (oven drying method).
View Article and Find Full Text PDFNarra J
December 2024
Department of Animal Production and Technology, Faculty of Animal Science, Institut Pertanian Bogor, Bogor, Indonesia.
Previous studies of IIA-1A5 have shown its potential as a probiotic in modulating gut microbiota and providing health benefits; however, its effects during pregnancy remain underexplored. The aim of this study was to assess the safety of fermented milk enriched with IIA-IA5 in pregnant mice. An experimental study was conducted at Universitas Andalas, Padang, Indonesia.
View Article and Find Full Text PDFMicrob Biotechnol
January 2025
Fonterra Microbiome Research Centre, University College Cork, Cork, Ireland.
Advancing microbiome-gut-brain axis science requires systematic, rational and translational approaches to bridge the critical knowledge gaps currently preventing full exploitation of the gut microbiome as a tractable therapeutic target for gastrointestinal, mental and brain health. Current research is still marked by many open questions that undermine widespread application to humans. For example, the lack of mechanistic understanding of probiotic effects means it remains unclear why even apparently closely related strains exhibit different effects in vivo.
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