Genotyping of isolated from animals using 25S ribosomal DNA and ALT repeats polymorphism in repetitive sequence.

Background And Purpose: is the most prevalent species isolated from animals. Candidiasis can be systemic in animals or may affect a single organ, such as the mouth, urinary tract, and skin. The aim of the present study was to determine the genetic diversity of isolated from different animals and investigate the presence of a relationship between host specificity and genetic typing of .

Materials And Methods: For the purpose of the study, DNA extraction was performed on 27 clinical isolates of obtained from animals. Subsequently, they were subjected to 25S ribosomal DNA amplification and ALT repeats in repetitive sequences (RPSs). The minimum inhibitory concentrations of fluconazole, ketoconazole, clotrimazole, nystatin, amphotericin B, and caspofungin were determined using the microdilution method based on the Clinical and Laboratory Standards Institute M27-S4 standard.

Results: Out of 27 strains, 11, 6, 5, and 5 cases were recognized as genotypes A (40.8%), E (22.2%), B (18.5%), and C (18.5%), respectively, through amplification using AS-I, which revealed 17 different types of . By combining the two typing methods, 27 strains were finally divided into 22 genotypes.

Conclusion: Different genotypes showed genetic diversity among the strains isolated from animal sources. The results revealed no special genotype relationship according to the host, anatomical source of isolation, and antifungal susceptibility.