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Liposome permeability probed by laser light scattering. | LitMetric

Liposome permeability probed by laser light scattering.

Biochem Mol Biol Educ

Department of Chemistry, Willamette University, Salem, Oregon, 97301.

Published: May 2019

We have developed a laboratory project in which students prepare liposomes, expose them to hyperosmotic and hypoosmotic solutions, and follow the resulting shrinking and swelling (respectively) with laser light scattering. Each light intensity transient can be fit to an exponential decline or rise, with the decay constant (k) and the amplitude (ΔV ) being indirectly related to the kinetics and thermodynamics (respectively) of transmembrane osmotic flux. Students vary the experimental system by changing the types and concentrations of osmolytes such as alcohols, amides, and salts. Students then compare how these changes alter the rate and extent of osmotic flux. This upper division biochemistry laboratory project is a challenging and rewarding one that exposes students to a biomolecule (lipid) and a spectroscopic technique (laser spectroscopy) that are not commonly used in the undergraduate laboratory setting. © 2019 International Union of Biochemistry and Molecular Biology, 47(3):239-246, 2019.

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Source
http://dx.doi.org/10.1002/bmb.21226DOI Listing

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