AI Article Synopsis
- Human embryonic stem cells showed quick and permanent movement of NANOG and OCT4 from the nucleus to the cytoplasm after just 30 minutes of stretching, while Sox2 remained unchanged.
- The movement was driven by biophysical signals relayed from cell surface integrins to the nuclear transport mechanism, without the influence of outside factors.
- When using E-CADHERIN-coated surfaces, even with limited integrin interaction, stretching still caused all three transcription factors to move quickly from the nucleus to the cytoplasm, suggesting important implications for understanding early development and bioengineering in stem cell research.
Article Abstract
Human embryonic stem cells subjected to a one-time uniaxial stretch for as short as 30-min on a flexible substrate coated with Matrigel experienced rapid and irreversible nuclear-to-cytoplasmic translocation of NANOG and OCT4, but not Sox2. Translocations were directed by intracellular transmission of biophysical signals from cell surface integrins to nuclear CRM1 and were independent of exogenous soluble factors. On E-CADHERIN-coated substrates, presumably with minimal integrin engagement, mechanical strain-induced rapid nuclear-to-cytoplasmic translocation of the three transcription factors. These findings might provide fundamental insights into early developmental processes and may facilitate mechanotransduction-mediated bioengineering approaches to influencing stem cell fate determination.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428113 | PMC |
| http://dx.doi.org/10.1093/intbio/zyz003 | DOI Listing |
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