The Type IV Secretion System of ICE1: Formation of a Conjugative Pilus in .

The dispersal of mobile genetic elements and their gene cargo relies on type IV secretion systems (T4SS). In this work the ICE1 Tra-type T4SS nanomachine, encoded in the publicly available genome of ATCC 23270, was characterized in terms of its organization, conservation, expression and mating bridge formation. Twenty-one conjugative genes grouped in four genetic clusters encode the ICE1 T4SS, containing all the indispensable functions for the formation and stabilization of the pili and for DNA processing. The clusters' organization resembles that of other mobile genetic elements (such as plasmids and integrative and conjugative elements-ICEs). Sequence conservation, genetic organization and distribution of the system in the genomes of other sequenced spp. suggests that the ICE1 T4SS could mediate the lateral gene transfer between related bacteria. All ICE1 T4SS genes are transcriptionally active and expressed from four independent operons. The transcriptional levels of selected marker genes increase in response to Mitomycin C treatment, a DNA damage elicitor that has acknowledged stimulatory effects on excision rates and gene expression of other ICEs, including ICE1. Using a tailor-made pilin-antiserum against ICE1 T4SS TraA pilin and epifluorescence microscopy, the presence of the conjugative pili on the cell surface of could be demonstrated. Additionally, immunodetection assays, by immunogold, allowed the identification of pili-like extracellular structures. Together, the results obtained in this work demonstrate that the ICE1 T4SS is phylogenetically conserved within the taxon, is expressed at mRNA and protein levels in the type strain, and produces a pili-like structure of extracellular and intercellular localization in this model acidophile, supporting its functionality. Additional efforts will be required to prove conjugation of the ICE1 or parts of this element through the cognate T4SS.