A carbapenem-resistant strain was isolated in Turkey in 2012 and and genes were observed in this strain. The aim of this study was to investigate transferability of plasmid bearing in and to use whole-genome sequencing in order to understand the genetic context of plasmid. strain was used as donor in conjugation experiments. Antibiotic susceptibility profile of selected transconjugant was determined. Plasmid was isolated from transconjugant colony and was named as pKPT. Complete sequencing of the pKPT was conducted using a next-generation sequencing. Annotation of the contigs was performed using the Geneious R9, followed by finding open reading frames (ORFs) with selected web-based tools. BLAST analysis was performed at the NCBI BLAST server to determine genes showing more than 90% similarity with these ORFs. Results of antibiotic susceptibility test showed that transconjugant colony was resistant to ampicillin/sulbactam, piperacillin, and piperacillin/tazobactam. The pKPT plasmid had a length of 45,217 bp and an average G + C content of 49%. Blast analysis revealed that pKPT was included in the IncL/M incompatibility group. The pKPT was found to contain within Tn transposon without any other antibiotic resistance gene.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1556/030.66.2019.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Global identification and regulatory network analysis reveal the significant roles of lncRNAs during anther and pollen development in Arabidopsis.
Plant Cell Rep
January 2025
Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, China.
A high-throughput sequencing identified 1283 lncRNAs in anthers at different stages in Arabidopsis and their relationship with protein-coding genes and miRNAs during anther and pollen development were analyzed. Long non-coding RNAs (lncRNAs) are important regulatory molecules involved in various biological processes. However, their roles in male reproductive development and interactions with miRNAs remained elusive.
View Article and Find Full Text PDFMulti-sample long-read nanopore sequencing of Agabus bipustulatus (Coleoptera: Dytiscidae: Agabinae) mitogenome produces effectively reliable data for downstream analyses.
J Insect Sci
January 2025
ZooLab, Department of Biodiversity and Ecology, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Bratislava, Slovakia.
Mitochondrial genomes are a rich source of data for various downstream analyses such as population genetics, phylogeny, and systematics. Today it is possible to assemble rapidly large numbers of mitogenomes, mainly employing next-generation sequencing and third-generation sequencing. However, verification of the correctness of the generated sequences is often lacking, especially for noncoding, length-variable parts.
View Article and Find Full Text PDFGenomics costing tool: considerations for improving cost-efficiencies through cross scenario comparison.
Front Public Health
January 2025
Technical Advice and Partnership Department, The Global Fund to Fight AIDS, Tuberculosis and Malaria, Geneva, Switzerland.
Next-generation sequencing (NGS) is crucial for monitoring and investigating infectious disease outbreaks, providing essential data for public health decisions. The COVID-19 pandemic has significantly expanded pathogen sequencing and bioinformatics capacities worldwide, creating an opportunity to leverage these advancements for other pathogens with pandemic and epidemic potential. In response to the need for a systematic cost estimation approach for sustainable genomic surveillance, particularly in low- and middle-income countries, five institutions collaborated to develop the genomics costing tool (GCT).
View Article and Find Full Text PDFOxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart disease.
Front Microbiol
January 2025
German Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, Germany.
Background: Early life gut microbiota is known to shape the immune system and has a crucial role in immune homeostasis. Only little is known about composition and dynamics of the intestinal microbiota in infants with congenital heart disease (CHD) and potential influencing factors.
Methods: We evaluated the intestinal microbial composition of neonates with CHD ( = 13) compared to healthy controls (HC, = 30).
Real-world clinical impact of plasma cell-free DNA metagenomic next-generation sequencing assay.
Infect Control Hosp Epidemiol
January 2025
Department of Pathology and Laboratory Medicine, University of California, Los Angeles, CA, USA.
Objective: To describe the real-world clinical impact of a commercially available plasma cell-free DNA metagenomic next-generation sequencing assay, the Karius test (KT).
Methods: We retrospectively evaluated the clinical impact of KT by clinical panel adjudication. Descriptive statistics were used to study associations of diagnostic indications, host characteristics, and KT-generated microbiologic patterns with the clinical impact of KT.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!