Rampant environmental pollution is the most ubiquitous concern of current world. A sustainable panacea to overarching contamination of water-borne pathogens demands cheap and eco-friendly oriented research. Solar energy is effortlessly accessible in most of the weather conditions and can be used for water decontamination. In this context, Solar Water Disinfection (SWADIS) appears to be feasible solution. Herein we are reporting newly developed Carbon nanoparticles (CNP) which shows absorption of light in broad region extending from Ultraviolet-Visible (UV) to Infrared Spectroscopy (IR). This CNP with pronounced photothermal effect has been used for SWADIS. Photothermal effect of plasmonic nanomaterials has massive potential and has exploited for disinfection of water. Moving towards practical device design we have developed an efficient CNP based Multipurpose Solar Pasteurizer (MSP) and Nano-Solar Pasteurizer (NSP) which can efficiently perform the SWADIS. Result shows that upon irradiation under natural solar radiation pasteurizers can thermally inactivate the bacteria. The system proves to be able to perform 100% bacterial inactivation in sunny days. We also conducted bacterial inactivation experiments by simulating 106 CFU mL concentration of E. coli in water to mimic field conditions. Results are evident that pasteurizers achieved 100% bacterial inactivation within period of ˜45 min under sunlight.
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http://dx.doi.org/10.1016/j.jhazmat.2019.02.052 | DOI Listing |
Electrophoresis
January 2025
National Institute for Nuclear, Chemical and Biological Protection, Kamenna, Czech Republic.
Timely identification of highly pathogenic bacteria is crucial for efficient mitigation of the connected harmful health effects. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) of intact cells enables fast identification of the microorganisms based on their mass spectrometry protein fingerprint profiles. However, the MALDI-TOF MS examination must be preceded by a time-demanding cultivation of the native bacteria to isolate representative cell samples to obtain indicative fingerprints.
View Article and Find Full Text PDFNat Commun
January 2025
Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, USA.
The bacterium Bacillus subtilis undergoes asymmetric cell division during sporulation, producing a mother cell and a smaller forespore connected by the SpoIIQ-SpoIIIA (or Q-A) channel. The two cells differentiate metabolically, and the forespore becomes dependent on the mother cell for essential building blocks. Here, we investigate the metabolic interactions between mother cell and forespore using genome-scale metabolic and expression models as well as experiments.
View Article and Find Full Text PDFNat Commun
January 2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Wall teichoic acids (WTAs) from the major Gram-positive foodborne pathogen Listeria monocytogenes are peptidoglycan-associated glycopolymers decorated by monosaccharides that, while not essential for bacterial growth, are required for bacterial virulence and resistance to antimicrobials. Here we report the structure and function of a bacterial WTAs rhamnosyltransferase, RmlT, strictly required for L. monocytogenes WTAs rhamnosylation.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Key Laboratory of Coal Conversion and New Carbon Materials of Hubei Province & Institute of Advanced Materials and Nanotechnology, School of Chemistry and Chemical Engineering, School of Medicine, Wuhan University of Science and Technology, Wuhan, China.
Bacterial infection of skin wounds leads to serious health problems, including skin defects, inflammatory pain, and even death. To meet the requirements for successful treatment of complicated wounds, a multifunctional dressing is thus highly desirable. In this work, a thermosensitive hydrogel dressing (HBCA) exhibiting injectability, adaptiveness and mild photothermal antibacterial activity was developed for effective infected wound treatment.
View Article and Find Full Text PDFBiotechnol Lett
December 2024
Jiangsu Key Laboratory for Pathogens and Ecosystems, College of Life Sciences, Nanjing Normal University, No.1 Wenyuan Rd., Xixia District, Nanjing, 210023, Jiangsu, People's Republic of China.
Recombineering (recombination-mediated genetic engineering) is a powerful strategy for bacterial genomic DNA and plasmid DNA modifications. CoS-MAGE improved over MAGE (multiplex automated genome engineering) by co-electroporation of an antibiotic resistance repair oligo along with the oligos for modification of the Escherichia coli chromosome. After several cycles of recombineering, the sub-population of mutants were selected among the antibiotic resistant colonies.
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