Enhancement of Synthesis of Bacillus cereus Enterotoxin Using a Sac-Culture Technique.

Synthesis of Bacillus cereus enterotoxin was enchanced when the organism was grown by the sac-culture method. Of the three other major extracellular proteins examined, only lecithinase synthesis was enhanced. Advantages of the sac-culture method are that it helps simplify purification of B. cereus enterotoxin, and that while being simpler and more reduced in scale than that of a fermenter culture, it still yields the same enterotoxin levels on a per ml basis.