Intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus and clones derived from these hybrids and backcrosses to Saccharum spp. were used to study the transmission of E. arundinaceus chromosomes by genomic in situ hybridization (GISH). True hybrid progenies were precisely identified using PCR with a primer pair, AGRP52/53. The results showed that AGRP52/53 was an E. arundinaceus-specific primer pair and could be used as molecular marker to assist breeding. EaHN92, a 364 bp E. arundinaceus-specific tandem repeat satellite DNA sequence, was cloned from the E. arundinaceus clone HN92-105 with AGRP52/53, and was localized on sub-telomeric regions of all E. arundinaceus chromosomes. YCE06-61, a BC progeny, had 7 E. arundinaceus chromosomes and its progenies had approximately 1-6 E. arundinaceus chromosomes. The number of E. arundinaceus chromosomes in true hybrids appeared as Gaussian distribution in 3 cross combinations. In addition, GISH detected intergeneric chromosome translocation in a few progenies. Hence, screening clones containing approximately 1-2 E. arundinaceus chromosomes without translocation could be used for sorting and sequencing E. arundinaceus chromosomes. This study provides a method for breeders to select true hybrid progenies between Saccharum spp. and E. arundinaceus, which will accelerate this intergeneric hybridization breeding.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385618 | PMC |
| http://dx.doi.org/10.1038/s41598-019-38710-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Male-biased recombination at chromosome ends in a songbird revealed by precisely mapping crossover positions.
G3 (Bethesda)
September 2024
Department of Biology, Lund University, 22362 Lund, Sweden.
Recombination plays a crucial role in evolution by generating novel haplotypes and disrupting linkage between genes, thereby enhancing the efficiency of selection. Here, we analyze the genomes of 12 great reed warblers (Acrocephalus arundinaceus) in a 3-generation pedigree to identify precise crossover positions along the chromosomes. We located more than 200 crossovers and found that these were highly concentrated toward the telomeric ends of the chromosomes.
View Article and Find Full Text PDFRecView: an interactive R application for locating recombination positions using pedigree data.
BMC Genomics
November 2023
Department of Biology, Lund University, Lund, 22362, Sweden.
Background: Recombination reshuffles alleles at linked loci, allowing genes to evolve independently and consequently enhancing the efficiency of selection. This makes quantifying recombination along chromosomes an important goal for understanding how selection and drift are acting on genes and chromosomes.
Results: We present RecView, an interactive R application and its homonymous R package, to facilitate locating recombination positions along chromosomes or scaffolds using whole-genome genotype data of a three-generation pedigree.
Evidence of Site-Specific and Male-Biased Germline Mutation Rate in a Wild Songbird.
Genome Biol Evol
November 2023
Department of Biology, Lund University, Lund, Sweden.
Germline mutations are the ultimate source of genetic variation and the raw material for organismal evolution. Despite their significance, the frequency and genomic locations of mutations, as well as potential sex bias, are yet to be widely investigated in most species. To address these gaps, we conducted whole-genome sequencing of 12 great reed warblers (Acrocephalus arundinaceus) in a pedigree spanning 3 generations to identify single-nucleotide de novo mutations (DNMs) and estimate the germline mutation rate.
View Article and Find Full Text PDFGenome-Wide Identification, Characterization and Expression Analysis of Plant Nuclear Factor (NF-Y) Gene Family Transcription Factors in spp.
Genes (Basel)
May 2023
Division of Crop Improvement, Indian Council of Agricultural Research-Sugarcane Breeding Institute, Coimbatore 641007, India.
Article Synopsis
- The study focuses on the Plant Nuclear Factor (NF-Y), which consists of three subfamilies (NF-YA, NF-YB, and NF-YC) that play different roles in plant development and stress responses.
- In sugarcane, researchers identified 51 NF-Y genes and mapped them across all 10 chromosomes, revealing conserved protein domains and distinct phylogenetic relationships with related species like sorghum.
- Expression analysis indicated that certain NF-Y genes are significantly involved in drought tolerance, with higher expression levels found in roots and leaves of both sugarcane and its drought-tolerant wild relative.
Evaluation of genome size and phylogenetic relationships of the complex species.
3 Biotech
November 2022
The Key Laboratory for Crop Production and Intelligent Agriculture of Yunnan Province, Kunming, 650201 Yunnan China.
Unlabelled: " complex" is a hypothetical group of species, which is supposed to be involved in the origin of modern sugarcane, and displays large genomes and complex chromosomal alterations. The utilization of restricted parents in breeding programs of modern cultivated sugarcane has resulted in a genetic blockage, which controlled its improvement because of the limited genetic diversity. The use of wild relatives is an effective way to broaden the genetic composition of cultivated sugarcane.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!