AI Article Synopsis
- Aspergillus oryzae is a key microorganism in food and bio industries, so understanding its regulatory mechanisms is essential for its safe application.
- Researchers identified a new gene, kpeA, which is thought to enhance kojic acid production and is linked to key processes in fungal growth, particularly in conidia production.
- The study found that disrupting kpeA results in longer hyphae, reduced conidia production, and increased kojic acid output, suggesting kpeA plays a vital role in regulating these functions at the transcriptional level.
Article Abstract
Aspergillus oryzae is an important microorganism in the bio- and food industries; therefore, understanding the mechanism underlying its secondary metabolism regulation is important for ensuring its safe use. Here, we screened a novel Zn(II)-Cys-type protein-encoding gene, AO090003001186, designated as kpeA (kojic acid production enhancement A), from an A. oryzae disruption mutant library of transcriptional regulators. kpeA is highly conserved among filamentous fungi and encodes a protein with Zn(II)-Cys motif located in the middle of the sequence. Phylogenetic analysis revealed that KpeA was classified into a distal group compared to other fungal Zn(II)-Cys-type transcriptional regulators. A Cys to Ala substitution mutant of KpeA showed identical phenotype to the kpeA disruption strain, confirming that KpeA is novel type Zn(II)-Cys binding protein. Colonies of the kpeA disruption strain (ΔkpeA) had longer aerial hyphae and showed decreased conidia production. Microscopic analysis suggested that the reduced vesicle size and conidial head formation in ΔkpeA strain account for the decreased conidia production. Transcriptional levels of brlA and downstream abaA and wetA were decreased in ΔkpeA strain. Moreover, ΔkpeA strain produced 6-fold more kojic acid than the control strains, and the expression of kojR and kojA was increased in ΔkpeA strain. Therefore, KpeA is a novel Zn(II)-Cys-type protein likely involved in conidiation and kojic acid production at the transcriptional level.
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| http://dx.doi.org/10.1016/j.fgb.2019.02.004 | DOI Listing |
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