Identification and Mapping of a New Soybean Male-Sterile Gene, .

The use of sterility is common in plants and multiple loci for hybrid sterility have been identified in crops such as rice. In soybean, fine-mapping and research on the molecular mechanism of male sterility is limited. Here, we identified a male-sterile soybean line, which produces larger, abnormal pollen grains that stain poorly with I-KI. In an inheritance test, all plants were fertile and the and populations conformed with the expected segregation ratio of 3:1 (fertility:sterility) ( = 0.82) and showed a 1:2:0 ratio of homozygous fertile: heterozygous fertile: homozygous sterile genotypes ( = 0.73), suggesting that the sterility was controlled by a single recessive gene (designated ""). Bulked segregant analysis showed that almost all single-nucleotide polymorphisms (SNPs; 95.92%) were distributed on chromosome 13 and 868 SNPs (95.81%) were distributed in the physical region of Chromosome 13.21877872 to Chromosome 13.22862641. Genetic mapping revealed that was flanked by and dCAPS-1 with genetic distances of 0.6 and 1.8 cM, respectively. The order of the consensus markers and known sterility genes was: Satt146 - (5.0 cM) - - (2.5 cM) - Satt030 - (15.3 cM) - - (5.0 cM) - Satt149 - (39.5 cM) - - (0.6 cM) - - (14.1 cM) - Satt516 (7.5 cM) - - (16.3 cM) - Satt595. These results suggest that is a newly identified male-sterility gene, which represents an alternative genetic resource for developing a hybrid seed production system for soybean.