Reprogramming of the cambium regulators during adventitious root development upon wounding of storage tap roots in radish ( L.).

Cambium contains a stem cell population that produces xylem and phloem tissues in a radial direction during the secondary growth stage. The growth of many storage roots, including in the radish, L., also depends on cambium. Interestingly, we observed numerous adventitious roots (ARs) emerging from the cambia of cut surfaces when the bases of radish storage tap roots were removed. Previous studies in Arabidopsis showed that the pathway regulates AR initiation and meristem establishment in an auxin-dependent manner Here, we provide evidence indicating the evolutionary conservation of the pathway during the AR development in radishes. Additionally, we found that expression of two cambium regulators, and , is induced in the cambium regions that are connected to emerging ARs via vascularization. Both AR formation and genes associated with this were induced by exogenous auxin. Our research suggests that some key cambium regulators might be reprogrammed to aid in the AR development in concert with the pathway.This article has an associated First Person interview with the first author of the paper.