AI Article Synopsis
- This study focuses on Pseudomonas aeruginosa Sequence Type 235 in Colombia, highlighting its ability to acquire resistance genes, particularly those related to carbapenem resistance, which poses a significant infection threat in healthcare settings.
- In a study conducted across five ICUs, 58 P. aeruginosa isolates were collected, with nearly half showing carbapenem resistance; molecular analysis revealed specific genes associated with resistance within certain isolates.
- The research uncovers a novel chromosomal feature involving a double insertion of Tn4401b in a new genomic island (PAGI-17), showcasing the organism's genomic adaptability and complexity in how it spreads antibiotic resistance.
Article Abstract
Background: Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in bla-positive P. aeruginosa ST235 in Colombia.
Results: In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the bla and bla genes, respectively. The four bla-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2″)-Ia, two Tn402-like with ant(3″)-Ia and bla and two Tn4401b with bla. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring bla were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background.
Conclusion: This is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381643 | PMC |
| http://dx.doi.org/10.1186/s12866-019-1418-6 | DOI Listing |
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