AI Article Synopsis
- The study aimed to assess the occurrence of Chagas disease in seronegative individuals in Brazil's endemic areas, given concerns over current testing methods.
- A total of 2,157 individuals were recruited from a public health database, with 2,091 providing samples for testing; out of these, 149 (7.1%) tested seronegative for T. cruzi.
- The findings indicated that true seronegative infections are rare, with no positive PCR results in seronegative individuals, suggesting that current antibody screening methods for blood supply are adequate.
Article Abstract
The diagnosis of Chagas disease is based on the detection of Trypanosoma cruzi (T. cruzi)-specific antibodies. Nonetheless, there is concern about the sensitivity of current serological assays due to reports of T. cruzi PCR positivity among seronegative individuals. The aim of this study was to evaluate if T. cruzi seronegative infections occur in endemic areas. We recruited 2,157 individuals that were identified as having Chagas disease in a public health system database of an endemic region in Brazil. All participants were interviewed and 2,091 had a sample collected for serological and PCR testing. From these, 149 (7.1%) had negative serological results. PCR was positive in 610 samples (31.4%) of the 1,942 seropositive samples but in none of the 149 samples from seronegative participants. True T. cruzi seronegative infections seem to be rare (95% CI 0-3.7) and should not be a concern for blood supply, which relies on antibody screening.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376933 | PMC |
| http://dx.doi.org/10.1590/S1678-9946201961011 | DOI Listing |
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