Exploring the rheological properties of intracellular materials is essential for understanding cellular and subcellular processes. Optical traps have been widely used for physical manipulation of micro and nano objects within fluids enabling studies of biological systems. However, experiments remain challenging as it is unclear how the probe particle's mobility is influenced by the nearby membranes and organelles. We use liposomes (unilamellar lipid vesicles) as a simple biomimetic model of living cells, together with a trapped particle rotated by optical tweezers to study mechanical and rheological properties inside a liposome both theoretically and experimentally. Here, we demonstrate that this system has the capacity to predict the hydrodynamic interaction between three-dimensional spatial membranes and internal probe particles within submicron distances, and it has the potential to aid in the design of high resolution optical micro/nanorheology techniques to be used inside living cells.
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http://dx.doi.org/10.1002/jbio.201900022 | DOI Listing |
Int J Biol Macromol
January 2025
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China. Electronic address:
The controlled release active packaging film represents a novel technology that always can effectively slow down the release of active agents, extending their efficacy. Mucorracemosus Fresenius (MF) mycelium was prepared and used as an adsorption carrier to load clove essential oil (CEO). The CEO/MF complexes were incorporated into the starch/polyvinyl alcohol (Starch/PVA) matrix to develop active films.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Anhui Key Laboratory of Advanced Building Materials, Anhui Jianzhu University, Hefei 230022, China.
A styrene-glycidylmethacrylate-1-allyl-3-vinylimidazole epoxy functionalized ionomer (EFI) was synthesized, and the EFI and carbon nanotubes (CNTs) were co-introduced into poly(lactide)/poly(butylene-adipate-co-terephtalate) (PLA/PBAT) blends to fabricate high performance composites with excellent mechanical properties, fatigue-resistance and dielectric properties. It is revealed that EFI can improve the interaction force between PLA and PBAT by inducing the interfacial crosslink reaction, thereby improving the melt strength of the samples. EFI can also refine the dispersion of CNT in the composites owing to the non-covalent force between EFI and CNT, promote the formation of filler network inside composites, which is demonstrated by DMA and rheological test results.
View Article and Find Full Text PDFFood Chem
January 2025
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China.
The effect of pores distribution on the multi-scale structure, enzyme accessibility, and pasting properties of the waxy maize starch granules with the same degree of hydrolysis were examined. Increased maltogenic α-amylase (MA) dosage obviously increased the shallow pores number and the roughness, whereas extended time increased the holes depth. Despite achieving the same hydrolysis degree and specific surface area, samples with numerous shallow holes exhibited a higher mass fractal dimension, a lower, peak viscosity, final viscosity and setback.
View Article and Find Full Text PDFRev Sci Instrum
January 2025
School of Perceptual Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
This paper presents a flat-type piezoelectric motor utilizing in-plane vibration modes. Two piezoelectric ceramic plates in combination with a brass metal sheet were used to construct the stator. The superposition of two second order in-plane vibration modes can generate a traveling-wave inside the stator.
View Article and Find Full Text PDFLab Chip
January 2025
Institute of Translational Medicine, Department of Health Sciences and Technology, ETH Zürich, 8092 Zürich, Switzerland.
Proteases, an important class of enzymes that cleave proteins and peptides, carry a wealth of potentially useful information. Devices to enable routine and cost effective measurement of their activity could find frequent use in clinical settings for medical diagnostics, as well as some industrial contexts such as detecting on-line biological contamination. In particular, devices that make use of readouts involving magnetic particles may offer distinct advantages for continuous sensing because material they release can be magnetically captured downstream and their readout is insensitive to optical properties of the sample.
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