Actinomadura craniellae sp. nov., isolated from a marine sponge in the South China Sea.

Int J Syst Evol Microbiol

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.

Published: April 2019

A novel marine actinomycete, designated LHW63021, was isolated from a marine sponge, genus Craniella, collected in the South China Sea. A polyphasic approach was applied to characterize the taxonomic position of this strain. The strain was found to have scarce aerial mycelia that differentiated into spore chains. The cell-wall hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid. Glucose, galactose, mannose and madurose were found in the whole-cell hydrolysates. The dominant polar lipids were phosphatidylinositol and diphosphatidylglycerol. MK-9(H6) and MK-9(H8) were the predominant menaquinones. The major fatty acids were iso-C16 : 0, iso-C18 : 0, 10-methyl C17 : 0 and C18 : 1 ω9c. The DNA G+C content based on the draft genome sequence was 72.0 mol%. 16S rRNA gene sequence analysis indicated that strain LHW63021 was a member of the genus Actinomadura and had the highest similarity to Actinomadura echinospora DSM 43163 (97.3 %). Phylogenetic trees supported their close relationship. The average nucleotide identity and digital DNA-DNA hybridization values between the whole genome sequences of strain LHW63021 and A. echinospora DSM 43163 were 79.13 and 23.20 %, respectively. The evidence from the polyphasic study shows that strain LHW63021 represents a novel species of the genus Actinomadura, for which the name Actinomadura craniellae sp. nov. is proposed. The type strain is LHW63021 (=DSM 106125=CCTCC AA 2018015).

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http://dx.doi.org/10.1099/ijsem.0.003295DOI Listing

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A novel marine actinomycete, designated LHW63021, was isolated from a marine sponge, genus Craniella, collected in the South China Sea. A polyphasic approach was applied to characterize the taxonomic position of this strain. The strain was found to have scarce aerial mycelia that differentiated into spore chains.

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