Prevalence and antimicrobial resistance of species isolated from diarrheal patients in Ahvaz, southwest Iran.

Introduction: Shigellosis is a significant global human health problem, and is in charge of almost 165 million cases of this disease annually, of whom 163 million cases are in developing countries and 1.5 million cases are in developed countries. The main aims of the current survey were to identify spp. isolated from diarrheal patients by conventional biochemical tests, determine the antimicrobial susceptibility profiles by disk diffusion method, and detect the gene using the PCR assay.

Methods: The bacterial isolates were identified as spp. by microbiological tests and were serogrouped by the slide agglutination test. Antimicrobial susceptibility testing was performed using the disk diffusion method. PCR was performed to detect the gene.

Results: The strains were isolated from 522 patients with various diarrhea, including bloody diarrhea (3%), mucoid plus bloody diarrhea (1.9%), mucoid diarrhea (3.2%), and watery diarrhea (3.2%). Overall, 69 (13.2%) isolates were positive for spp., of which 34 (49.3%) serotypes were identified as , 22 (31.9%) serotypes were identified as , 9 (13%) serotypes were identified as , and 4 (5.8%) serotypes were identified as . Antibiotic susceptibility tests revealed that the highest resistance percentage was related to ampicillin (82%) and trimethoprim-sulfamethoxazole (77%), and ciprofloxacin and ceftriaxone were the best antibiotics against isolates.

Conclusion: We concluded that spp. can be considered as an etiological agent of diarrhea in southwest Iran. Since the drug resistance pattern of differs geographically and over time within a country, continuous and regular surveillance program is necessary.