The occurrence of drug hypersensitivity reactions (DHRs) following administration of low molecular weight (LMW) drugs is an important health concern. However, in vivo animal models which could be used as tools for the prediction of DHRs are lacking. As a result, research has focused on development of in vitro tools for predicting DHRs. In this study a novel human in vitro pre-clinical skin explant test was used to predict T cell-mediated hypersensitivity responses induced by LMW drugs. Responses in the skin explant test for 12 LMW drugs associated with T cell-mediated hypersensitivity in the clinic (abacavir, amoxicillin, carbamazepine, diclofenac, lamotrigine, lapatinib, lumiracoxib, nevirapine, ofloxacin, phenytoin, propranolol, sulfamethoxazole) were compared with responses for 5 drugs with few/no reports of T cell-mediated hypersensitivity reactions (acetaminophen, cimetidine, flecainide, metformin, verapamil). Changes in skin histology following in vitro exposure to the drugs as well as T cell proliferation and interferon gamma (IFNγ) production were studied. The results of the skin explant assays showed a good positive correlation (r = 0.77, p < .001) between the test outcome (prediction of positive or negative) and the clinical classification of the tested drugs. The T cell proliferation assay showed a correlation of r = 0.60 (p < .01) and the IFNγ assay r = 0.51 (p < .04). The data suggest that the skin explant model could be a useful tool to predict the potential of LMW drugs to induce DHRs.
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http://dx.doi.org/10.1016/j.taap.2019.02.005 | DOI Listing |
Am Fam Physician
January 2025
University of Kansas Medical Center, Kansas City.
Acute rhinosinusitis causes more than 30 million patients to seek health care per year in the United States. Respiratory tract infections, including bronchitis and sinusitis, account for 75% of outpatient antibiotic prescriptions in primary care. Sinusitis is a clinical diagnosis; the challenge lies in distinguishing between the symptoms of bacterial and viral sinusitis.
View Article and Find Full Text PDFPLoS One
January 2025
Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Background: According to Rome IV, reflux hypersensitivity (RH) represents a novel form of functional esophageal disorder. This study was designed to compare the clinical features of three types of endoscopic-negative heartburn: RH, nonerosive reflux disease (NERD), and functional heartburn (FH).
Methods: Patients with heartburn in a medical center from 01/01/2017 to 10/31/2021 were included.
J Clin Exp Dent
December 2024
Triplet Biotechnology Solutions, Inc., Bauru, SP, Brazil.
Background: Dentin hypersensitivity affects a significant portion of the world's population, causing pain and negatively impacting oral health and quality of life This clinical study investigated the effectiveness of a desensitizing fluoride silicon-rich dental gel associated with a calcium booster.
Material And Methods: In this single-blind, randomized, parallel-group clinical trial, forty-eight volunteers diagnosed with dentin hypersensitivity and qualified to participate were randomized into 2 groups: (1) fluoride silicon-rich dental gel (REFX Technology)/calcium booster (Si/Ca protocol); (2) Pumice stone diluted in saline. A draw was carried out between the right and left sides of the patients so that either a prophylaxis with the dental gel associated with calcium or with fine pumice stone diluted in saline was carried out.
Cureus
December 2024
Conservative Dentistry and Endodontics, SRM Kattankulathur Dental College and Hospital, SRM Institute of Science and Technology (SRMIST), Chengalpattu, IND.
With over two millennia of history, traditional Chinese acupuncture ranks among the most renowned forms of supplementary and other healthcare. The use of acupuncture releases endorphins and serotonin, two naturally occurring painkillers, into the nervous system and stimulates it. It also modifies how pain signals are processed and perceived.
View Article and Find Full Text PDFJ Integr Plant Biol
January 2025
School of Advanced Agricultural Sciences, Peking University, Beijing, 100871, China.
Heat stress (HS) at the reproductive stage detrimentally affects crop yields and seed quality. However, the molecular mechanisms that protect reproductive processes in plants under HS remain largely unknown. Here, we report that Acetylation Lowers Binding Affinity 3 (ALBA3) is crucial for safeguarding male fertility against HS in Arabidopsis.
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