Nematode chemosensation is a vital component of their host-seeking behavior. The globally important phytonematode perceives and responds (via sensory organs such as amphids and phasmids) differentially to various chemical cues emanating from the rhizosphere during the course of host finding. However, compared with the free-living worm , the molecular intricacies behind the plant nematode chemotaxis are a yet-unexploited territory. In the present study, four putative chemosensory genes of , namely, , , , and were molecularly characterized. mRNA was found to be expressed in the cell bodies of amphidial neurons and phasmids of . , , , and transcripts were highly expressed in early life stages of , consistent with a role of these genes in host recognition. Functional characterization of , , , and via RNA interference revealed behavioral defects in and perturbed attraction to host roots in Pluronic gel medium. Knockdown of , , , and resulted in defective chemotaxis of to various volatile compounds (alcohol, ketone, aromatic compound, ester, thiazole, pyrazine), nonvolatiles of plant origin (carbohydrate, phytohormone, organic acid, amino acid, phenolic), and host root exudates in an agar-Pluronic gel-based assay plate. In addition, ascaroside-mediated signaling was impeded by downregulation of chemosensory genes. This new information that behavioral response in is modulated by specific olfactory genes can be extended to understand chemotaxis in other nematodes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1094/MPMI-08-18-0226-R | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative analyses of odorant binding protein orthologues in three sympatric Tomicus bark beetles provide insights into functional differentiation of OBPs to ecologically relevant odorants and insecticides.
Int J Biol Macromol
December 2024
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China. Electronic address:
Three Tomicus bark beetles (T. yunnanensis, T. brevipilosus and T.
View Article and Find Full Text PDFAberrant Positions of the Chemosensory Neurons in the Neurotransmitter-Release Mutant .
Int J Mol Sci
December 2024
Department of Genetics, Silberman Institute of Life Science, Edmond J. Safra Campus, The Hebrew University of Jerusalem, Jerusalem 9112001, Israel.
Secretion of neurotransmitter- and neuropeptide-containing vesicles is a regulated process orchestrated by multiple proteins. Of these, mutants, defective in the and genes, responsible for neurotransmitter and neuropeptide release, respectively, are routinely used to elucidate neural and circuitry functions. While these mutants result in severe functional deficits, their neuroanatomy is assumed to be intact.
View Article and Find Full Text PDFIndependent Genetic Mapping Experiments Identify Diverse Molecular Determinants of Host Adaptation in a Generalist Herbivore.
Mol Ecol
December 2024
Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Interactions between plants and herbivores promote evolutionary change. Studying the evolution of herbivore mechanisms aimed to cope with different host plant species is a critical intersection between evolutionary biology and sustainable pest management. Generalist herbivores are of particular interest, as hybridization between genetically distinct populations can increase the standing genetic variation and therefore the adaptive potential of the species.
View Article and Find Full Text PDFSpecialized chemosensory signals elicit innate social behaviors in individuals of several vertebrate species, a process that is mediated via the accessory olfactory system (AOS). The AOS comprising the peripheral sensory vomeronasal organ has evolved elaborate molecular and cellular mechanisms to detect chemo signals. To gain insight into the cell types, developmental gene expression patterns, and functional differences amongst neurons, we performed single-cell transcriptomics of the mouse vomeronasal sensory epithelium.
View Article and Find Full Text PDFAdult single-nucleus neuronal transcriptomes of insulin signaling mutants reveal regulators of behavior and learning.
Cell Genom
December 2024
Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. Electronic address:
Gene expression in individual neurons can change during development to adulthood and can have large effects on behavior. Additionally, the insulin/insulin-like signaling (IIS) pathway regulates many of the adult functions of Caenorhabditis elegans, including learning and memory, via transcriptional changes. We used the deep resolution of single-nucleus RNA sequencing to define the adult transcriptome of each neuron in wild-type and daf-2 mutants, revealing expression differences between L4 larval and adult neurons in chemoreceptors, synaptic genes, and learning/memory genes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!