AI Article Synopsis
- - The study aims to explore how DNA methylation in monocytes might influence the development of acute diabetic Charcot foot (CF) in patients with Type 2 diabetes (T2D).
- - Researchers analyzed DNA methylation patterns in monocytes from 54 T2D patients, comparing those with acute CF, those with similar neuropathy but no CF, and those without neuropathy, utilizing advanced bisulfite sequencing techniques.
- - Findings revealed significant differences in DNA methylation of monocytes in CF patients, with several key genes linked to monocyte migration and inflammatory regulation showing altered methylation and related gene expression.
Article Abstract
Aim: To assess whether DNA methylation of monocytes play a role in the development of acute diabetic Charcot foot (CF).
Patients & Methods: We studied the whole methylome (WM) of circulating monocytes in 18 patients with Type 2 diabetes (T2D) and acute CF, 18 T2D patients with equivalent neuropathy and 18 T2D patients without neuropathy, using the enhanced reduced representation bisulfite sequencing technique.
Results & Conclusion: WM analysis demonstrated that CF monocytes are differentially methylated compared with non-CF monocytes, in both CpG-site and gene-mapped analysis approaches. Among the methylated genes, several are involved in the migration process during monocyte differentiation into osteoclasts or are indirectly involved through the regulation of inflammatory pathways. Finally, we demonstrated an association between methylation and gene expression in cis- and trans-association.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2217/epi-2018-0144 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!