In this study 200 E. coli strains were isolated and identified from 95 fecal, 96 urinary and 9 vaginal specimens. The pathogenic species isolated from feces were 61.05% of all identified E. coli strains. This percentage was 26.04% for urinary specimens. The distribution of E. coli serotypes was determined. Mostly E. coli O111 K58 B4 strain was isolated from the pathological materials such as 20% in feces, 12.5% in urine, 11.11% in vaginal secretions. The sex distribution of isolated E. coli strains from urine was 77.08% for female patients and 22.92% male patients. We can say that E. coli species which have been isolated and identified from pathological materials are susceptible to seftriaxon 73.5% and sefotaxime 72.5%, but they have been readily gaining resistance against gentamicin and nalidixic acid. Broad spectrum antibiotics and chemotherapeutic agents are widely used in the treatment of E. coli infections. However medical therapy doesn't always produce good result. Because E. coli strains have been gaining resistance to chemotherapeutic agents and antibiotics. So, it is suggested that the most effective antibiotic should be determined by antibiogram and used.
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Food Sci Nutr
January 2025
Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences Sylhet Agricultural University Sylhet Bangladesh.
The emergence of antibiotic-resistant microorganisms has made antimicrobial resistance a global issue, and milk is a potential source for the propagation of resistant bacteria causing zoonotic diseases. Subclinical mastitis (SCM) cases, often overlooked and mixed with normal milk in dairy farms, frequently involve , which can spread through contaminated milk. We conducted this study to determine the prevalence of virulence genes, antibiotic resistance genes (ARGs), antimicrobial susceptibility, and the genetic relatedness of multidrug-resistant (MDR) Shiga toxin-producing (STEC) isolated from SCM milk.
View Article and Find Full Text PDFJapanese encephalitis (JE) is a mosquito-borne infectious disease caused by the Japanese encephalitis virus (JEV). There is currently no effective treatment for JE, and all approved Japanese encephalitis vaccine products originated from the JEV genotype III (GIII). In recent years, JEV genotype I (GI) has gradually replaced GIII as the dominant genotype, and a new symptom of peripheral nerve injury (PNI) caused by JEV NX1889 strain has attracted wide attention, in which JEV envelope (E) protein may be involved in early peripheral nerve injury.
View Article and Find Full Text PDFFront Antibiot
April 2024
Transmission, Reservoir and Diversity of Pathogens Unit, Institut Pasteur, Les Abymes, France.
Introduction: This study aimed to understand the origin and to explain the maintenance of extended-spectrum β-lactamase (ESBL) isolated from food-producing animals in a third-generation cephalosporin (3GC)-free farm.
Methods: Culture and molecular approaches were used to test molecules other than 3GC such as antibiotics (tetracycline and oxytetracycline), antiparasitics (ivermectin, flumethrin, fenbendazol, and amitraz), heavy metal [arsenic, HNO, aluminum, HNO, cadmium (CdSO), zinc (ZnCl), copper (CuSO), iron (FeCl), and aluminum (AlSO)], and antioxidant (butylated hydroxytoluene) as sources of selective pressure. Whole-genome sequencing using short read (Illumina™) and long read (Nanopore™) technologies was performed on 34 genomes.
ACS Synth Biol
January 2025
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
2'-Fucosyllactose (2'-FL) is the most abundant human milk oligosaccharides (HMOs). 2'-FL exhibits great benefits for infant health, such as preventing infantile diarrhea and promoting the growth of intestinal probiotics. The microbial cell factory technique has shown promise for the massive production of 2'-FL.
View Article and Find Full Text PDFAnal Chem
January 2025
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.
Lipid A, a well-known saccharolipid, acts as the inner lipid-glycan anchor of lipopolysaccharides in Gram-negative bacterial cell membranes and functions as an endotoxin. Its structure is composed of two glucosamines with β(1 → 6) linkages and various fatty acyl and phosphate groups. The lipid A structure can be used for the identification of bacterial species, but its complexity poses significant structural characterization challenges.
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