This study evaluated the expression of genes involved in the concentration of Ca in precursor osteoblast-like cell, MC3T3-E1 subjected to stretching stimuli. Transient receptor potential vanilloid 4 (Trpv4) gene expression, the factor that is activated by stretch stimulation and enables inflow of Ca from the extracellular space, was not affected as a result of stretch stimulation; conversely, the expression of sodium-calcium exchanger 1 (Ncx1) gene involved in outflow of intracellular Ca increased, depending on stimulation intensity. Localization of Ca correlated with the positioning of the endoplasmic reticulum, and intracellular Ca decreased in inverse proportion to the intensity of the stretching force. These results suggest that stretch stimulation activates intracellular Ca elimination rather than Ca uptake before osteoblast differentiation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483915 | PMC |
| http://dx.doi.org/10.1292/jvms.18-0766 | DOI Listing |
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