AI Article Synopsis
- Respiratory syncytial virus (RSV) neutralizing antibodies (NAbs) are crucial for protection against RSV, highlighting the need for a reliable assay to measure them.
- An imaging-based micro-neutralization assay has been developed that is effective for RSV subgroup A and can also work for subgroup B, demonstrating high reproducibility and low inter-assay variation.
- This cost-effective assay can be implemented in labs globally, improving standardization and supporting the evaluation of new RSV vaccine candidates.
Article Abstract
Respiratory syncytial virus-specific neutralizing antibodies (RSV NAbs) are an important marker of protection against RSV. A number of different assay formats are currently in use worldwide so there is a need for an accurate and high-throughput method for measuring RSV NAbs. We describe here an imaging-based micro-neutralization assay that has been tested on RSV subgroup A and can also be adapted for RSV subgroup B and different sample types. This method is highly reproducible, with inter-assay variations for the reference antiserum being less than 10%. We believe this assay can be readily established in many laboratories worldwide at relatively low cost. Development of an improved, high-throughput assay that measures RSV NAbs represents a significant step forward for the standardization of this method internationally as well as being critical for the evaluation of novel RSV vaccine candidates in the future.
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| http://dx.doi.org/10.3791/59025 | DOI Listing |
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